When procedures are in place for ensuring that originals are archived, and image manipulation steps are reported, scientists not only follow good laboratory practices, but also avoid ethical issues associated with postprocessing and protect their labs from any future allegations of scientific misconduct.
Also, when procedures are in place for correct acquisition of images, the extent of postprocessing is minimized or eliminated. These procedures include color balancing for brighfield images , keeping tonal values within the dynamic range of the detector, frame averaging to eliminate noise typically in fluorescence imaging , use of the highest bit depth when a choice is available, flatfield correction, and archiving of the image in a nonlossy format not JPEG.
When postprocessing is necessary, the commonly used applications for correction include Photoshop, and ImageJ, but a free program GIMP can also be used. These corrections are explained in step-by-step procedures in the chapter that follows. Authors: Jerry Sedgewick 1.
Jerry Sedgewick 1. Full text PDF Related articles. Abstract Augmentation of digital images is almost always a necessity in order to obtain a reproduction that matches the appearance of the original.
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